This work describes a new protocol to assess the ecotoxicity of pollutants, including emerging contaminants such as nanomaterials, using the marine bacterium Vibrio anguillarum. This method allows for the determination of the LC50, or mortality, the concentration that causes a 50% decrease in bacteria culturability, after a 6 h exposure.
Rotini, A., Manfra, L., Spanu, F., Pisapia, M., Cicero, A. M., Migliore, L. Ecotoxicological Method with Marine Bacteria Vibrio anguillarum to Evaluate the Acute Toxicity of Environmental Contaminants. J. Vis. Exp. (123), e55211, doi:10.3791/55211 (2017).