Journal
/
生物化学
/
Quantification of Bacterial Histidine Kinase Autophosphorylation Using a Nitrocellulose Binding Assay
JoVE 杂志
生物化学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 生物化学
Quantification of Bacterial Histidine Kinase Autophosphorylation Using a Nitrocellulose Binding Assay

Quantification of Bacterial Histidine Kinase Autophosphorylation Using a Nitrocellulose Binding Assay

8,398 Views

•

09:17 min

•

January 11, 2017

DOI:

10.3791/55129-v

DOI:
10.3791/55129-v

•

09:17 min
January 11, 2017

•

2 Views
, ,
1Department of Chemistry,Stony Brook University, 2Department of Physiology and Biophysics,Stony Brook University

Explore More Videos

Bacterial Histidine KinaseAutophosphorylationQuantificationNitrocellulose Binding AssayTwo-component SignalingPost-translational ModificationRadioactive LigandsHigh-throughputKinetic ParametersProtein-protein InteractionsFiltration96-well Dot-plot ApparatusGamma-32P ATPPhosphoric Acid

Read Article

Cite this Article

Fischer, J., Johnson, R. A., Boon, E. Quantification of Bacterial Histidine Kinase Autophosphorylation Using a Nitrocellulose Binding Assay. J. Vis. Exp. (119), e55129, doi:10.3791/55129 (2017).

Download .ris file

Copy

Share Video

.

Copy