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发育生物学
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Production and Administration of Therapeutic Mesenchymal Stem/Stromal Cell (MSC) Spheroids Primed in 3-D Cultures Under Xeno-free Conditions
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发育生物学
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JoVE 杂志 发育生物学
Production and Administration of Therapeutic Mesenchymal Stem/Stromal Cell (MSC) Spheroids Primed in 3-D Cultures Under Xeno-free Conditions

Production and Administration of Therapeutic Mesenchymal Stem/Stromal Cell (MSC) Spheroids Primed in 3-D Cultures Under Xeno-free Conditions

DOI:
10.3791/55126-v

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11:05 min

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March 18, 2017

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, , ,
1Department of Biology,University of Mary Hardin-Baylor, 2Department of Pediatrics,University of Texas Medical Branch, 3Multi-Organ Support Technology Task Area,U.S. Army Institute of Surgical Research, 4Internal Medicine,Texas A&M University Health Science Center

Chapters

  • 00:05Title
  • 01:25MSC Expansion and Preparation of Activated MSC Spheroids in 3-D Hanging Drop Cultures
  • 04:50Intraperitoneal Delivery of Intact Spheroids
  • 08:02Results: Spheroids Produced Using MSC Hanging Drop Cultures
  • 09:31Conclusion

Summary

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The therapeutic potential of mesenchymal stem/stromal cells (MSCs) is well-documented, however the best method of preparing the cells for patients remains controversial. Herein, we communicate protocols to efficiently generate and administer therapeutic spherical aggregates or 'spheroids' of MSCs primed under xeno-free conditions for experimental and clinical applications.

Tags

Mesenchymal Stem/stromal CellsMSC SpheroidsXeno-free ConditionsCell RetentionCell SurvivalTherapeutic Efficacy3D CulturePrimingCell HarvestingCell CountingSpheroid FormationSpheroid Harvesting

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