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Isolamento rápido de BMPR-IB + adiposo-derivados de células estromais para uso em um Defeito Modelo Cura calota craniana
JoVE 杂志
发育生物学
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JoVE 杂志 发育生物学
Rapid Isolation of BMPR-IB+ Adipose-Derived Stromal Cells for Use in a Calvarial Defect Healing Model
DOI:

09:07 min

February 24, 2017

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Chapters

  • 00:05Title
  • 01:16Harvesting and Isolation of ASCs
  • 03:19FACS Sorting for BMPR-1B Positive Cells
  • 05:39Creation of Calvarial Defects and Application of ACS-containing Scaffold
  • 07:03Results: Bone Regeneration in Response to BMPR-1B-positive ASC
  • 08:02Conclusion

Summary

自动翻译

Adipose-derived stromal cells may be useful for engineering new tissue from a patient's own cells. We present a protocol for the isolation of a subpopulation of human adipose-derived stromal cells (ASCs) with increased osteogenic potential, followed by application of the cells in an in vivo calvarial healing assay.

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