Journal
/
神经科学
/
The CApillary FEeder Assay Measures Food Intake in Drosophila melanogaster
JoVE 杂志
神经科学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 神经科学
The CApillary FEeder Assay Measures Food Intake in Drosophila melanogaster

The CApillary FEeder Assay Measures Food Intake in Drosophila melanogaster

DOI:
10.3791/55024-v

•

07:42 min

•

March 17, 2017

•

, , , , , ,
1Institute of Zoology,Albertus-Magnus University of Cologne, 2Department of Veterinary Biomedical Sciences, Western College of Veterinary Medicine,University of Saskatchewan

Chapters

  • 00:05Title
  • 00:38CAFE Assay Vial Preparation
  • 01:35Fly Preparation
  • 02:52Liquid Food Preparation
  • 03:24Assembly and CApillary FEeder Assay Performance
  • 06:14Results: Food Preferences Clearly Ascertained
  • 07:04Conclusion

Summary

自动翻译

自动翻译

The CApillary FEeder (CAFE) assay is a simple, budget-friendly, highly reliable method for investigating mechanisms underlying food intake. Used with the highly versatile genetic model organism Drosophila melanogaster, it provides a powerful means of gaining new insights into regulatory mechanisms of food intake.

Tags

CApillary FEeder AssayDrosophila MelanogasterFood IntakeMetabolic ResearchFeeding BehaviorLiquid FoodMicrocapillariesFly CultureExperimental SetupCO2 AnesthesiaFly WeightFood ConsumptionLong-term Experiment

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

Single Sensillum Recordings in the Insects Drosophila melanogaster and Anopheles gambiae

Paired Nanoinjection and Electrophysiology Assay to Screen for Bioactivity of Compounds using the Drosophila melanogaster Giant Fiber System

Labeling of Single Cells in the Central Nervous System of Drosophila melanogaster

Optogenetic Stimulation of Escape Behavior in Drosophila melanogaster

Determination of the Spontaneous Locomotor Activity in Drosophila melanogaster

Straightforward Assay for Quantification of Social Avoidance in Drosophila melanogaster

Conditions Affecting Social Space in Drosophila melanogaster

A Behavioral Assay for Mechanosensation of MARCM-based Clones in Drosophila melanogaster

High Throughput Assay to Examine Egg-Laying Preferences of Individual Drosophila melanogaster

A Simple Way to Measure Alterations in Reward-seeking Behavior Using Drosophila melanogaster

Read Article