Journal
/
生物化学
/
SorLA and CLC:CLF-1-dependent Downregulation of CNTFRα as Demonstrated by Western Blotting, Inhibition of Lysosomal Enzymes, and Immunocytochemistry
JoVE 杂志
生物化学
This content is Free Access.
JoVE 杂志 生物化学
SorLA and CLC:CLF-1-dependent Downregulation of CNTFRα as Demonstrated by Western Blotting, Inhibition of Lysosomal Enzymes, and Immunocytochemistry

SorLA and CLC:CLF-1-dependent Downregulation of CNTFRα as Demonstrated by Western Blotting, Inhibition of Lysosomal Enzymes, and Immunocytochemistry

DOI:
10.3791/55019-v

•

10:16 min

•

January 06, 2017

•

,
1Department of Biomedicine,Aarhus University

Chapters

  • 00:05Title
  • 00:55Western Blotting of CNTFRα Lysates
  • 03:21Immunocytochemistry in Combination with Lysosomal Inhibitors
  • 06:00Western Blot-detection of the pSTAT3 Levels
  • 08:17Results: Demonstration of CNTFRα-downregulation by Western Blotting, and Inhibition of Lysosomal Enzymes, and Immunocytochemistry
  • 09:43Conclusion

Summary

自动翻译

自动翻译

The present protocol describes how Western blotting and immunocytochemistry combined with inhibition of lysosomal enzymes can be used to demonstrate the downregulation of Ciliary Neurotrophic Factor Receptor-α (CNTFRα), which is conveyed by the interaction between Cytokine-like Factor-1 (CLF-1) and the endocytic receptor sorLA.

Tags

SorLACLC:CLF-1CNTFRWestern BlottingImmunocytochemistryLysosomal DegradationGPI-anchored ReceptorProtein TurnoverStable Cell LinesTransfectants

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

High-throughput Screening of Carbohydrate-degrading Enzymes Using Novel Insoluble Chromogenic Substrate Assay Kits

Identification of Plant Ice-binding Proteins Through Assessment of Ice-recrystallization Inhibition and Isolation Using Ice-affinity Purification

Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase

A Western Blotting Protocol for Small Numbers of Hematopoietic Stem Cells

A11-positive β-amyloid Oligomer Preparation and Assessment Using Dot Blotting Analysis

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition

Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy

Cortisol Measurement in Koala (Phascolarctos cinereus) Fur

Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

Read Article