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发育生物学
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Isolation and Expansion of Adult Canine Hippocampal Neural Precursors
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发育生物学
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JoVE 杂志 发育生物学
Isolation and Expansion of Adult Canine Hippocampal Neural Precursors

Isolation and Expansion of Adult Canine Hippocampal Neural Precursors

DOI:
10.3791/54953-v

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09:37 min

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November 29, 2016

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, , ,
1Regenerative Neuroscience Group,University of Sydney, 2Wellcome Trust Centre for Neuroimaging, Institute of Neurology,University College London

Chapters

  • 00:05Title
  • 00:44Brain Extraction
  • 02:38Hippocampal Dissection and Neural Precursor Cells Isolation
  • 04:38Neurosphere Culture and Passage
  • 06:32Adherent Culture of Neural Precursor Cells
  • 08:10Results: In Vitro Expansion and Immunocytochemistry of Adult Canine Hippocampal Neural Precursor Cells
  • 09:07Conclusion

Summary

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自动翻译

The canine brain is a valuable model in which to study adult neurogenesis. Presented here are protocols for isolating and expanding adult canine hippocampal neural precursor cells from primary brain tissue.

Tags

Neural Precursor CellsAdult Canine HippocampusAdult NeurogenesisHigher Mammal Animal ModelPost Mortem ExtractionBrain DissectionHippocampus IsolationCell Culture

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