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一种优化的协议分析和糖酵解线粒体呼吸的淋巴细胞
JoVE 杂志
免疫与感染
This content is Free Access.
JoVE 杂志 免疫与感染
An Optimized Protocol to Analyze Glycolysis and Mitochondrial Respiration in Lymphocytes
DOI:

08:40 min

November 21, 2016

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Chapters

  • 00:05Title
  • 01:04Tissue Processing
  • 03:28Magnetic B Cell and Naïve CD4+ T Cell Separation
  • 04:43Extracellular Flux Assay
  • 06:09Results: Representative Oxygen Consumption Rate (OCR) and Extracellular Acidification Rate (ECAR) Analyses
  • 07:48Conclusion

Summary

自动翻译

The study of metabolism is becoming increasingly relevant to immunological research. Here, we present an optimized method for measuring glycolysis and mitochondrial respiration in mouse splenocytes, and T and B lymphocytes.

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