Journal
/
/
对于神经化学标记用biocytin填充和加工部分的免疫染色
JoVE 杂志
神经科学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 神经科学
Immunostaining of Biocytin-filled and Processed Sections for Neurochemical Markers
DOI:

08:54 min

December 31, 2016

, , ,

Chapters

  • 00:05Title
  • 01:19Biocytin-filling during Electrophysiology
  • 03:31Staining of the First Primary Antibody and Biocytin
  • 04:58Staining of the Second Primary Antibody
  • 06:32Results: Successful CCK Staining 1 Week Following the Recovery of Biocytin Staining and CB1R Labeling
  • 07:56Conclusion

Summary

自动翻译

该协议提出了在使用生物胞素填充和随后的免疫组织化学后处理电生理记录修补神经元的形态回收的方法。我们表明,该染色和盖片厚生物胞素填充部分可以与第二初级抗体天或数月后进行复染。

Related Videos

Read Article