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简单而高效的生产和鼠标髓鞘少突胶质糖蛋白的纯化的实验性自身免疫性脑脊髓炎研究
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JoVE 杂志 免疫与感染
Simple and Efficient Production and Purification of Mouse Myelin Oligodendrocyte Glycoprotein for Experimental Autoimmune Encephalomyelitis Studies
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简单而高效的生产和鼠标髓鞘少突胶质糖蛋白的纯化的实验性自身免疫性脑脊髓炎研究

DOI:
10.3791/54727-v

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10:47 min

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October 27, 2016

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, ,
1Department of Microbiology and Immunology,Western University

Chapters

  • 00:05Title
  • 00:54Protein Induction
  • 02:03Harvesting MOGtag Protein
  • 03:42Protein Purification
  • 06:17Dialysis and Concentrating MOGtag Protein
  • 08:58Results: Evaluation of MOGtag Purity and Assessment of its Activity
  • 09:39Conclusion

Summary

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自动翻译

We describe a simple protocol using only basic lab equipment to generate and purify large quantities of a fusion protein that contains mouse Myelin Oligodendrocyte Glycoprotein. This protein can be used to induce experimental autoimmune encephalomyelitis driven by both T and B cells.

Tags

Mouse Myelin Oligodendrocyte GlycoproteinExperimental Autoimmune EncephalomyelitisMOG ProteinProtein ExpressionBL21 E. ColiIPTG InductionCell LysisProtein PurificationHexa-histidine TagNickel-nitrilotriacetic Acid (Ni-NTA) Affinity Chromatography

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