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방법 및 HCN1 TRIP8b 간의 단백질 - 단백질 상호 작용의 작은 분자 억제제 식별
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10:20 min
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November 11, 2016
DOI:
1Davee Department of Neurology and Clinical Neurosciences,Feinberg School of Medicine, Northwestern University, 2Center for Molecular Innovation and Drug Discovery,Northwestern University, 3Department of Pharmacology,Feinberg School of Medicine, Northwestern University, 4High Throughput Analysis Laboratory, Department of Molecular Biosciences,Northwestern University, 5Department of Physiology,Feinberg School of Medicine, Northwestern University
Summary
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HCN 채널과 보조 서브 유닛 사이의 상호 작용은 주요 우울 장애의 치료 대상으로 확인되었습니다. 여기서,이 단백질 - 단백질 상호 작용의 소분자 억제제를 식별하기위한 형광 편광 - 기반 방법이 제공된다.
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Cite this Article
Han, Y., Lyman, K. A., Clutter, M., Schiltz, G. E., Ismail, Q., Cheng, X., Luan, C., Chetkovich, D. M. Method for Identifying Small Molecule Inhibitors of the Protein-protein Interaction Between HCN1 and TRIP8b. J. Vis. Exp. (117), e54540, doi:10.3791/54540 (2016).
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