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Eiwitcomplex Affiniteit Capture uit Cryomilled zoogdiercellen
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
Protein Complex Affinity Capture from Cryomilled Mammalian Cells
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Eiwitcomplex Affiniteit Capture uit Cryomilled zoogdiercellen

14,460 Views

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10:37 min

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December 09, 2016

DOI:

10.3791/54518-v

DOI:
10.3791/54518-v

•

10:37 min
December 09, 2016

•

7 Views
, ,
1Laboratory of Cellular and Structural Biology,The Rockefeller University, 2Institute for Systems Genetics, Department of Biochemistry and Molecular Pharmacology,New York University School of Medicine

Summary

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Hier beschrijven we protocollen bij zoogdiercellen door solid-state frezen verstoren bij een cryogene temperatuur, produceren een cel uittreksel uit de resulterende cel poeder, en te isoleren eiwitcomplexen van rente door affiniteitsinvangmiddel upon-antilichaam gekoppeld micron-schaal paramagnetische kralen.

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Cite this Article

LaCava, J., Jiang, H., Rout, M. P. Protein Complex Affinity Capture from Cryomilled Mammalian Cells. J. Vis. Exp. (118), e54518, doi:10.3791/54518 (2016).

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