Protocols are described for the fabrication of degradable thermoresponsive hydrogels based on hydrazone cross-linking of polymeric oligomers on the bulk scale, microscale, and nanoscale, the latter for preparation of both gel nanoparticles and nanofibers.
Sivakumaran, D., Bakaic, E., Campbell, S. B., Xu, F., Mueller, E., Hoare, T. Fabricating Degradable Thermoresponsive Hydrogels on Multiple Length Scales via Reactive Extrusion, Microfluidics, Self-assembly, and Electrospinning. J. Vis. Exp. (134), e54502, doi:10.3791/54502 (2018).