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Preparazione e consegna di microcristalli proteina nella fase cubica lipidica per Serial femtosecondi Cristallografia

JoVE 杂志
生物化学

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JoVE 杂志生物化学

Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography

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Preparazione e consegna di microcristalli proteina nella fase cubica lipidica per Serial femtosecondi Cristallografia

11,043 Views

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09:09 min

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September 20, 2016

DOI:

10.3791/54463-v

DOI:

10.3791/54463-v

•

09:09 min

September 20, 2016

•

9 Views

Andrii Ishchenko², Vadim Cherezov², Wei Liu

¹The Bridge Institute,University of Southern California, ²Department of Chemistry,University of Southern California, ³School of Molecular Sciences, Center for Applied Structural Discovery at the Biodesign Institute,Arizona State University

Tags

Protein Microcrystals Lipidic Cubic Phase Serial Femtosecond Crystallography Membrane Protein Structure Protein Reconstitution Sample Preparation Syringe Handling Precipitant Solution Protein-laden LCP Teflon Spherules Sample Sealing

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