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القياس الكمي لمستويات حمض الريتينويك النسبية في E8.5 الأجنة وNeurosphere الثقافات باستخدام F9 نادر-Lacz القائم على خلية مراسل الفحص
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Quantitative Measurement of Relative Retinoic Acid Levels in E8.5 Embryos and Neurosphere Cultures Using the F9 RARE-Lacz Cell-based Reporter Assay
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القياس الكمي لمستويات حمض الريتينويك النسبية في E8.5 الأجنة وNeurosphere الثقافات باستخدام F9 نادر-Lacz القائم على خلية مراسل الفحص

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09:49 min

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September 06, 2016

DOI:

10.3791/54443-v

DOI:
10.3791/54443-v

•

09:49 min
September 06, 2016

•

1 Views
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1Center for Advanced Biotechnology and Medicine,Rutgers University, 2Department of Neuroscience and Cell Biology,Rutgers University

Summary

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Methods to accurately measure retinoic acid (RA) levels in small amounts of tissue do not exist. This protocol describes the easy, quantitative measurement of relative RA levels in E8.5 embryos and neurospheres using an RA reporter cell line.

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Cite this Article

Ababon, M. R., Li, B. I., Matteson, P. G., Millonig, J. H. Quantitative Measurement of Relative Retinoic Acid Levels in E8.5 Embryos and Neurosphere Cultures Using the F9 RARE-Lacz Cell-based Reporter Assay. J. Vis. Exp. (115), e54443, doi:10.3791/54443 (2016).

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