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تصميم واستخدام ومنخفضة التكلفة، الآلي Morbidostat عن التكيف تطور البكتيريا تحت المضادات الحيوية اختيار الدواء
JoVE 杂志
遗传学
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JoVE 杂志 遗传学
Design and Use of a Low Cost, Automated Morbidostat for Adaptive Evolution of Bacteria Under Antibiotic Drug Selection
DOI:

10:50 min

September 27, 2016

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Chapters

  • 00:05Title
  • 00:46Assembly of the Morbidostat
  • 04:13Pretesting and Running the Morbidostat
  • 06:23On the Chip Growth Experiment
  • 07:38Results: Evolution of Antibiotic Drug Resistance using a Morbidostat
  • 09:31Conclusion

Summary

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We describe a low cost, configurable morbidostat that enables the characterization of antibiotic drug resistance by dynamically adjusting the drug concentration. The device can be integrated with a multiplexed microfluidic platform. The approach can be scaled up for laboratory antibiotic drug resistance studies.

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