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Bir Kullanarak Modeli Proteinler içine Kuralsız Amino Asit kalıntı özgü Ortaklığın<em> Escherichia coli</em> Hücre içermeyen Transkripsiyon-çeviri Sistemi
JoVE 杂志
生物学
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JoVE 杂志 生物学
Residue-specific Incorporation of Noncanonical Amino Acids into Model Proteins Using an Escherichia coli Cell-free Transcription-translation System
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Bir Kullanarak Modeli Proteinler içine Kuralsız Amino Asit kalıntı özgü Ortaklığın<em> Escherichia coli</em> Hücre içermeyen Transkripsiyon-çeviri Sistemi

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11:47 min

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August 01, 2016

DOI:

10.3791/54273-v

DOI:
10.3791/54273-v

•

11:47 min
August 01, 2016

•

6 Views
, , , , , ,
1Department of Experimental Physics,Saarland University, 2Institute of Chemistry,Technische Universität Berlin, 3School of Physics and Astronomy,University of Minnesota

Summary

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An easy-to-use, cell-free expression protocol for the residue-specific incorporation of noncanonical amino acid analogs into proteins, including downstream analysis, is presented for medical, pharmaceutic, structural and functional studies.

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Cite this Article

Worst, E. G., Exner, M. P., De Simone, A., Schenkelberger, M., Noireaux, V., Budisa, N., Ott, A. Residue-specific Incorporation of Noncanonical Amino Acids into Model Proteins Using an Escherichia coli Cell-free Transcription-translation System. J. Vis. Exp. (114), e54273, doi:10.3791/54273 (2016).

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