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Una citometría de flujo simple método para medir la captación de glucosa y la expresión del transportador de la glucosa por monocitos subpoblaciones en sangre entera
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JoVE 杂志 免疫与感染
A Simple Flow Cytometric Method to Measure Glucose Uptake and Glucose Transporter Expression for Monocyte Subpopulations in Whole Blood
DOI:

06:28 min

August 12, 2016

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Chapters

  • 00:05Title
  • 00:43Monocyte and Monocyte Subpopulation Glucose Transporter 1 (Glut1) Cell Surface Detection
  • 02:07Monocyte Glucose Uptake Analysis
  • 03:11Data Acquisition and Analysis
  • 04:16Results: Representative Monocyte Subpopulation Glucose Uptake Analysis
  • 05:22Conclusion

Summary

自动翻译

Monocytes are integral components of the human innate immune system that rely on glycolytic metabolism when activated. We describe a flow cytometry protocol to measure glucose transporter expression and glucose uptake by total monocytes and monocyte subpopulations in fresh whole blood.

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