需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Basit Akım Sitometrisi Yöntemi Tüm Kan Monosit subpopulations için glikoz alımını ve Glikoz Transporter Anlatım Tedbir
16,240 Views
•
06:28 min
•
August 12, 2016
DOI:
1Centre for Biomedical Research,Macfarlane Burnet Institute for Medical Research and Public Health, 2Department of Infectious Diseases,Monash University, 3Department of Microbiology and Immunology,University of Melbourne, 4Department of Microbiology,The University of the West Indies, 5Division of Experimental Medicine, Department of Medicine,University of California, San Francisco, 6Department of Medicine,Monash University
Summary
Automatically generated
Monocytes are integral components of the human innate immune system that rely on glycolytic metabolism when activated. We describe a flow cytometry protocol to measure glucose transporter expression and glucose uptake by total monocytes and monocyte subpopulations in fresh whole blood.
Read Article
Cite this Article
Palmer, C. S., Anzinger, J. J., Butterfield, T. R., McCune, J. M., Crowe, S. M. A Simple Flow Cytometric Method to Measure Glucose Uptake and Glucose Transporter Expression for Monocyte Subpopulations in Whole Blood. J. Vis. Exp. (114), e54255, doi:10.3791/54255 (2016).
Copy