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Makrofaj koloni stimüle edici faktör farklılaştırılmış insan monosit türevli makrofajlar Kültür
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免疫与感染
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JoVE 杂志 免疫与感染
Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages

Makrofaj koloni stimüle edici faktör farklılaştırılmış insan monosit türevli makrofajlar Kültür

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06:46 min

June 30, 2016

DOI:

06:46 min
June 30, 2016

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Summary

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A protocol is presented for cell culture of macrophage colony-stimulating factor (M-CSF) differentiated human monocyte-derived macrophages. The protocol utilizes cryopreservation of monocytes coupled with their bulk differentiation into macrophages. Then harvested macrophages can then be seeded into culture wells at required cell densities for carrying out experiments.

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