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Chapters
- 00:05Title
- 01:11Murine Femur and Tibia Single Bone Marrow Suspesion Preparation
- 05:55Bone Marrow Cell (BMC) Culture
- 08:39Results: Representative Bone Marrow Dendritic Cell Culture Analyses
- 10:13Conclusion
Bone marrow cells cultured with granulocyte macrophage colony stimulating factor (GM-CSF) generate a heterogeneous culture containing macrophages and dendritic cells (DCs). This method highlights using MHCII and hyaluronan (HA) binding to differentiate macrophages from the DCs in the GM-CSF culture. Macrophages in this culture have many similarities to alveolar macrophages.
