需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Geração e identificação de GM-CSF macrófagos derivados e dendríticas células alveolares do tipo de ratinho de Medula Óssea
17,585 Views
•
11:05 min
•
June 25, 2016
DOI:
Summary
Automatically generated
Bone marrow cells cultured with granulocyte macrophage colony stimulating factor (GM-CSF) generate a heterogeneous culture containing macrophages and dendritic cells (DCs). This method highlights using MHCII and hyaluronan (HA) binding to differentiate macrophages from the DCs in the GM-CSF culture. Macrophages in this culture have many similarities to alveolar macrophages.
Read Article
Cite this Article
Dong, Y., Arif, A. A., Poon, G. F. T., Hardman, B., Dosanjh, M., Johnson, P. Generation and Identification of GM-CSF Derived Alveolar-like Macrophages and Dendritic Cells From Mouse Bone Marrow. J. Vis. Exp. (112), e54194, doi:10.3791/54194 (2016).
Copy