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Immunology and Infection

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JoVE Journal Immunology and Infection
Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa
 

Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa

Article DOI: 10.3791/54115-v • 11:31 min • June 30th, 2016
June 30th, 2016 •
1, 1, 1
1Division of Molecular Microbiology, College of Life Sciences, University of Dundee

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This article describes the high-throughput assay that has been successfully established to screen large libraries of small molecules for their potential ability to manipulate cellular levels of cyclic di-GMP in Pseudomonas aeruginosa, providing a new powerful tool for antibacterial drug discovery and compound testing.

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High-throughput Setup Screening Small Molecules Modulate C-di-GMP Signaling Pseudomonas Aeruginosa Bio-reporter Screen Cyclic-di-GMP Modulation Robust And Versatile Technique 3,500 Compounds 48 Hours Novel Therapies Immune System Existing Antibiotics Selective Pressure Lysogeny Broth LB Medium Incubate At 37 Degrees Celsius Shaking At 200 Rpm Optical Density At 600 Nanometers (OD 600) Spectrophotometer
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