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En strategi för att öka Justering och myelinisering av Dorsal Root ganglieneuroner
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
An Approach to Enhance Alignment and Myelination of Dorsal Root Ganglion Neurons

En strategi för att öka Justering och myelinisering av Dorsal Root ganglieneuroner

DOI:

09:48 min

August 24, 2016

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Chapters

  • 00:05Title
  • 00:41Preparation of Pre-stretched Anisotropic Surface
  • 02:22Isolation of DRG
  • 05:07Culture of DRG on Pre-stretched Surface
  • 06:12Co-culture of Schwann Cells with DRG Neurons on Pre-stretched Surface
  • 07:54Results: Co-cultured Schwann Cells with Aligned DRG Axons on Stretched vs. Unstretched PDMS Substrates
  • 08:42Conclusion

Summary

自动翻译

This protocol describes the isolation of dorsal root ganglion (DRG) neurons isolated from rats and the culture of DRG neurons on a static pre-stretched cell culture system to enhance axon alignment, with subsequent co-culture of Schwann Cells (SCs) to promote myelination.

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