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Chapters
- 00:05Title
- 01:09Epithelial Wound Healing in Organ-cultured Corneas
- 02:57Adenoiral Transduction of Organ-cultured Corneas
- 04:31Isolation of Limbal Cells and Maintenance of Stem Cell Enriched Cultures
- 06:28Adenoiral Transduction of Cultured Limbal Cells
- 07:55Results: Effects of Gene Therapy on Wound Healing and Stem Cell Marker Expression
- 10:25Conclusion
An example of adenoviral gene therapy in the human diabetic organ-cultured corneas is presented towards the normalization of delayed wound healing and markedly reduced epithelial stem cell marker expression in these corneas. It also describes the optimization of this process in stem cell-enriched limbal epithelial cultures.
