Journal
/
发育生物学
/
Adenoviral Gene Therapy for Diabetic Keratopathy: Effects on Wound Healing and Stem Cell Marker Expression in Human Organ-cultured Corneas and Limbal Epithelial Cells
JoVE 杂志
发育生物学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 发育生物学
Adenoviral Gene Therapy for Diabetic Keratopathy: Effects on Wound Healing and Stem Cell Marker Expression in Human Organ-cultured Corneas and Limbal Epithelial Cells

Adenoviral Gene Therapy for Diabetic Keratopathy: Effects on Wound Healing and Stem Cell Marker Expression in Human Organ-cultured Corneas and Limbal Epithelial Cells

9,010 Views

•

11:13 min

•

April 07, 2016

DOI:

10.3791/54058-v

DOI:
10.3791/54058-v

•

11:13 min
April 07, 2016

•

2 Views
, ,
1Eye Program, Board of Governors Regenerative Medicine Institute, Departments of Biomedical Sciences and Neurosurgery,Cedars-Sinai Medical Center, 2David Geffen School of Medicine,University of California, Los Angeles

Summary

Automatically generated

An example of adenoviral gene therapy in the human diabetic organ-cultured corneas is presented towards the normalization of delayed wound healing and markedly reduced epithelial stem cell marker expression in these corneas. It also describes the optimization of this process in stem cell-enriched limbal epithelial cultures.

Explore More Videos

Adenoviral Gene TherapyDiabetic KeratopathyWound HealingStem Cell Marker ExpressionHuman Organ-cultured CorneasLimbal Epithelial CellsEpithelial Wound HealingCorneal Stem Cell FunctionCorneal DiseasesStem Cell DysfunctionEpithelial DebridementCorneal HealingApoptotic Stromal Keratocytes

Read Article

Cite this Article

Kramerov, A. A., Saghizadeh, M., Ljubimov, A. V. Adenoviral Gene Therapy for Diabetic Keratopathy: Effects on Wound Healing and Stem Cell Marker Expression in Human Organ-cultured Corneas and Limbal Epithelial Cells. J. Vis. Exp. (110), e54058, doi:10.3791/54058 (2016).

Download .ris file

Copy

Share Video

.

Copy