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Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization
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医学
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JoVE 杂志 医学
Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization

Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization

DOI:
10.3791/53928-v

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06:01 min

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June 07, 2016

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, ,
1Department of Biological Science, Center for Colon Cancer Research,University of South Carolina, 2Department of Pathology,Chonnam National University Medical School

Chapters

  • 00:05Title
  • 00:37Tissue Preparation
  • 02:02Hybridization
  • 03:08Stringency Wash and Blocking
  • 03:55Primary Antibody Incubation and Development
  • 05:14Results: Stronger miRNA-489 Expression in Normal Tissues
  • 05:37Conclusion

Summary

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Here we present a protocol to detect miRNA expression in breast cancer patient samples using miRNA in situ hybridization.

Tags

Keywords: MiRNAIn Situ HybridizationBreast CancerProbe HybridizationWash Steps

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