With the increased use of transcription factor-specific ChIP-seq technology, techniques to validate potential regulatory regions are critical. The authors describe a rapid chick bioassay to validate the activity of regulatory sequences functioning during development.
Pira, C. U., Caltharp, S. A., Haro, E., Oberg, K. C. A Rapid In Vivo Bioassay for Developmentally Active Enhancers. J. Vis. Exp. (Pending Publication), e53882, doi: (2015).