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Epigenetische Umwandlung als eine sichere und einfache Methode, Insulin absondernden Zellen aus adulten Fibroblasten der Haut zu erhalten
JoVE 杂志
发育生物学
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JoVE 杂志 发育生物学
Epigenetic Conversion as a Safe and Simple Method to Obtain Insulin-secreting Cells from Adult Skin Fibroblasts
DOI:

08:45 min

March 18, 2016

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Chapters

  • 00:05Title
  • 00:50Skin Fibroblast Isolation
  • 01:53Fibroblast Culture and Plating for Epigenetic Conversion
  • 04:00Increase Cell Plasticity Using the De-methylating Agent 5-aza-CR
  • 05:31Pancreatic Differentiation Protocol
  • 06:47Results: Morphological and Functional Changes During Epigenetic Conversion
  • 08:02Conclusion

Summary

自动翻译

Here, a new method that allows the conversion of adult skin fibroblasts into insulin-secreting cells is presented. This technique is based on epigenetic conversion, does not involve the use of retroviral vectors nor the acquisition of a stable pluripotent state. It is therefore highly promising for translational medicine applications.

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