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Isolamento e caracterização de células individuais de embriões Zebrafish
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发育生物学
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JoVE 杂志 发育生物学
Isolation and Characterization of Single Cells from Zebrafish Embryos

Isolamento e caracterização de células individuais de embriões Zebrafish

14,188 Views

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09:25 min

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March 12, 2016

DOI:

10.3791/53877-v

DOI:
10.3791/53877-v

•

09:25 min
March 12, 2016

•

18 Views
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1Department of Cell Biology and Physiology,University of North Carolina at Chapel Hill, 2McAllister Heart Institute,University of North Carolina at Chapel Hill, 3Department of Pathology and Laboratory Medicine,University of North Carolina at Chapel Hill

Summary

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This protocol describes a method for isolating single cells from zebrafish embryos, enriching for cells of interest, capturing zebrafish cells in microfluidic based single cell multiplex systems, and assessing gene expression from single cells.

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Single Cell IsolationZebrafish EmbryosMicrofluidic-based Single-cell AnalysisGene Expression发育生物学Cell HeterogeneityCell DissociationCell PreparationChorion RemovalDe-yolkingCell WashingCell Resuspension

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Cite this Article

Samsa, L. A., Fleming, N., Magness, S., Qian, L., Liu, J. Isolation and Characterization of Single Cells from Zebrafish Embryos. J. Vis. Exp. (109), e53877, doi:10.3791/53877 (2016).

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