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发育生物学
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Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues
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发育生物学
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JoVE 杂志 发育生物学
Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues

Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues

7,955 Views

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13:03 min

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June 03, 2016

DOI:

10.3791/53874-v

DOI:
10.3791/53874-v

•

13:03 min
June 03, 2016

•

2 Views
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1INSERM UMR-910, Faculté de Médecine,Université Aix-Marseille, 2Institute of Physiological Chemistry,University Medical Center, Johannes Gutenberg University

Summary

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A fine tuning regulation of gene transcription underlies embryonic cell fate decision. Herein, we describe chromatin immunoprecipitation assays used to investigate epigenetic regulation of both cardiac differentiation of stem cells and cardiac development of mouse embryos.

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Epigenetic RegulationCardiac DifferentiationEmbryonic Stem CellsChromatin ImmunoprecipitationGene TranscriptionEmbryonic DevelopmentCardiac DevelopmentEpigenetic MarksDNAEmbryoid BodiesCross-linkingCell FixationCell LysisSonicationProtease Inhibitors

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Cite this Article

Jebeniani, I., Leschik, J., Puceat, M. Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues. J. Vis. Exp. (112), e53874, doi:10.3791/53874 (2016).

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