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发育生物学
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Pflegen Primäre Nasenepithelzellen von Kindern und umprogrammieren in induzierte pluripotente Stammzellen
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发育生物学
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JoVE 杂志 发育生物学
Cultivate Primary Nasal Epithelial Cells from Children and Reprogram into Induced Pluripotent Stem Cells
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Pflegen Primäre Nasenepithelzellen von Kindern und umprogrammieren in induzierte pluripotente Stammzellen

10,959 Views

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12:08 min

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March 10, 2016

DOI:

10.3791/53814-v

DOI:
10.3791/53814-v

•

12:08 min
March 10, 2016

•

3 Views
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1Pyrosequencing Core,Cincinnati Children’s Hospital, 2Division of Developmental Biology,Cincinnati Children’s Hospital, 3Division of Pulmonary Medicine,Seattle Children’s Hospital, 4Division of Asthma Research,Cincinnati Children’s Hospital

Summary

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This publication demonstrates methods for successful sampling and culture of nasal epithelial mucosa from children, and reprogramming these cells to induced Pluripotent Stem Cells (iPSCs).

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Cite this Article

Ulm, A., Mayhew, C. N., Debley, J., Khurana Hershey, G. K., Ji, H. Cultivate Primary Nasal Epithelial Cells from Children and Reprogram into Induced Pluripotent Stem Cells. J. Vis. Exp. (109), e53814, doi:10.3791/53814 (2016).

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