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Myeloid Cell Isolation from Mouse Skin and Draining Lymph Node Following Intradermal Immunization with Live Attenuated Plasmodium Sporozoites
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Myeloid Cell Isolation from Mouse Skin and Draining Lymph Node Following Intradermal Immunization with Live Attenuated Plasmodium Sporozoites

Myeloid Cell Isolation from Mouse Skin and Draining Lymph Node Following Intradermal Immunization with Live Attenuated Plasmodium Sporozoites

DOI:
10.3791/53796-v

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08:46 min

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May 18, 2016

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, , ,
1Unité de Biologie et Génétique du Paludisme,Institut Pasteur, 2Unité dImmunobiologie des Cellules Dendritiques,Institut Pasteur

Chapters

  • 00:05Title
  • 00:37Radiation-attentuated Sporozoite Isolation from Mosquito Salivary Glands
  • 02:07Sporozoite Injection
  • 03:13Skin and Draining Lymphs Node Dissection
  • 04:49Cell Isolation
  • 07:18Results: Intradermal RAS Injection Increases Myeloid Cell Migration to Mouse Ear Skin and dLN
  • 08:01Conclusion

Summary

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We describe here a protocol for isolating myeloid cells from mouse skin and draining lymph node following intradermal injection of Plasmodium sporozoites. Flow cytometry of collected cells provides a reliable assay to characterize the skin and draining lymph node inflammatory response to the parasite.

Tags

Keywords: Myeloid Cell IsolationMouse SkinDraining Lymph NodeIntradermal ImmunizationLive Attenuated Plasmodium SporozoitesMalariaInnate Immune ResponseRadiation-attenuated ParasitesMosquito Salivary GlandsSporozoite InjectionEar PinnaDermis

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