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HSV-medieret transgene ekspression af kimære konstruktioner at undersøge adfærdsmæssige funktion GPCR heteromerer i mus
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神经科学
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JoVE 杂志 神经科学
HSV-Mediated Transgene Expression of Chimeric Constructs to Study Behavioral Function of GPCR Heteromers in Mice

HSV-medieret transgene ekspression af kimære konstruktioner at undersøge adfærdsmæssige funktion GPCR heteromerer i mus

DOI:
10.3791/53717-v

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07:30 min

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July 09, 2016

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, ,
1Department of Psychiatry,Icahn School of Medicine at Mount Sinai, 2Department of Neurology,Icahn School of Medicine at Mount Sinai, 3Friedman Brain Institute,Icahn School of Medicine at Mount Sinai, 4Department of Physiology and Biophysics,Virginia Commonwealth University Medical School

Chapters

  • 00:05Title
  • 00:56Stereotaxic Surgery to Introduce HSV Chimeric Constructs
  • 03:32Head Twitch Response Experiment
  • 05:04Data Review
  • 05:33Results
  • 06:35Conclusion

Summary

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Denne artikel beskriver, hvordan at injicere virale vektorer i musen frontale cortex at teste adfærdsmæssige analyser, der kræver GPCR heteromere formation.

Tags

HSV-Mediated Transgene ExpressionChimeric ConstructsBehavioral FunctionGPCR HeteromersMiceVirus-mediated Gene TransferBehavioral PhenotypesNeuro PharmacologyPsycho PharmacologyG-protein Coupled ReceptorsMolecular Level InteractionPsychiatric DisordersSchizophreniaDepressionSeratonin 2a ReceptorMetabotropic Glutamate Receptor 2Mechanism Of ActionDrugs For Schizophrenia TreatmentAnesthesiaSurgical Plane Of AnesthesiaOphthalmic GelStereotactic FrameScalp IncisionPureit Clamps

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