需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Chapters
- 00:05Title
- 00:52Characterizing Early Elongation Using 4D (Time Lapse Z-stack) DIC Imaging
- 03:47Characterizing Late Elongation Using Flow Cytometry
- 08:23Results: 4D-DIC versus Flow Cytometry for Sizing Nematode Larvae
- 09:32Conclusion
Here we detail protocols specifically designed to monitor morphogenic defects that occur during early and late phases of embryonic elongation of the nematode Caenorhabditis elegans. Ultimately, these protocols are designed to identify genes that regulate these phases and to characterize their differential requirements along the antero-posterior axis of the embryo.
