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发育生物学
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Initiating Differentiation in Immortalized Multipotent Otic Progenitor Cells
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发育生物学
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JoVE 杂志 发育生物学
Initiating Differentiation in Immortalized Multipotent Otic Progenitor Cells

Initiating Differentiation in Immortalized Multipotent Otic Progenitor Cells

DOI:
10.3791/53692-v

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12:17 min

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January 02, 2016

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, , , ,
1Cell Biology & Neuroscience,Rutgers University

Chapters

  • 00:05Title
  • 00:49Maintaining Self-Renewal in IMOP Cells
  • 03:26Differentiating IMOP Cells into Sensory Epithelia
  • 05:56Differentiating IMOP-Derived Neurons
  • 09:46Results: Differentiation of IMOP Cells into Inner Ear Sensory Epithelia and Spiral Ganglion Neurons
  • 11:47Conclusion

Summary

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The current protocols to maintain immortalized multipotent otic progenitor (iMOP) cells and otic differentiation are described. Culture conditions and molecular markers that indicate differentiation into sensory epithelia and spiral ganglion neurons (SGN) are highlighted.

Tags

DifferentiationImmortalized Multipotent Otic Progenitor CellsInner Ear Cell TypesAuditory RegenerationCochlear Hair CellsAuditory NeuronsIn-vitro Cellular SystemIn-vivo Mammalian Animal Model SystemsCulture MediumIMOP CellsIncubateCarbon DioxidePassage CellsConical TubeOtospheres

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