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Inactivation of mTor: A Tool to Investigate Meiotic Progression and Translational Control During Bovine Oocyte Maturation
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September 24, 2015
DOI:
DOI:
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00:00 min
September 24, 2015
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1BVN Neustadt/Aisch, 2Faculty of Veterinary Medicine, Clinic for Obstetrics, Gynecology and Andrology of Large and Small Animals,Justus-Liebig-University Giessen, 3Reproductive Cell Biology Unit,Leibniz Institute for Farm Animal Biology, 4Reproductive Biochemistry Unit,Leibniz Institute for Farm Animal Biology
Summary
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The protocol presented here describes the inhibition of the Ser/Thr kinase mTor during IVM of bovine oocytes. This approach can facilitate the investigation of meiotic progression and translational control. It also contributes to the definition of developmental competence and the improvement of IVM conditions.
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Kreißelmeier, M., Mayer, S., Wrenzycki, C., Pöhland, R., Tomek, W. Inactivation of mTor: A Tool to Investigate Meiotic Progression and Translational Control During Bovine Oocyte Maturation. J. Vis. Exp. (Pending Publication), e53689, doi: (2015).
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