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JoVE Journal Neuroscience
Hele Mount Dissektion og Immunofluorescens af Voksen Mouse Cochlea
 
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Hele Mount Dissektion og Immunofluorescens af Voksen Mouse Cochlea

Article DOI: 10.3791/53561-v • 12:02 min • January 1st, 2016
January 1st, 2016 •
1, 1,2
1Department of Surgery, Division of Otolaryngology, Southern Illinois University, School of Medicine, 2Department of Pharmacology, Southern Illinois University, School of Medicine

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Vi præsenterer en metode til at isolere voksne organ Corti som tre intakte cochlear sving (apex, midten og bunden). Vi viser også en procedure for immunfarvning med fluorescens mærkede antistoffer. Tilsammen gør disse teknikker gør det muligt studiet af hårceller, støtteceller, og andre celletyper fundet i cochlea.

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Neurovidenskab organ Corti hårceller der støtter celler cochlea indre øre tidsmæssige knogle forbehandling immunhistokemi immunfarvning immunolabeling neurovidenskab
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