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A Behavioral Essai pour mécanosensibilité de marcm-fondé clones dans<em> Drosophila melanogaster</em
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
A Behavioral Assay for Mechanosensation of MARCM-based Clones in Drosophila melanogaster

A Behavioral Essai pour mécanosensibilité de marcm-fondé clones dans<em> Drosophila melanogaster</em

DOI:

05:48 min

December 30, 2015

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Chapters

  • 00:05Title
  • 00:48MARCM Fly Generation and Timed Egg-Laying
  • 02:11Heat Shock Induction
  • 02:54Behavioral Testing
  • 04:38Results: Lack of Response in Mechanosenstivive Mutants
  • 05:10Conclusion

Summary

自动翻译

In order to identify novel mutations affecting mechanosensation, we designed an assay that measures the behavioral response to tactile stimulation of fly bristles in mutant clones generated by the MARCM method. The combination of techniques allows for the identification of mechanosensitive mutations that would otherwise be lethal.

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