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Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation
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Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation

Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation

10,200 Views

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10:52 min

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January 06, 2016

DOI:

10.3791/53529-v

DOI:
10.3791/53529-v

•

10:52 min
January 06, 2016

•

7 Views
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1Department of Chemistry and Biology, Program in Molecular Science,Ryerson University

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RadiolabelingPhosphoinositidesHPLCFlow ScintillationYeastMammalian CellsInositolPerchloric AcidEDTACentrifugationSonicationLiquid CultureCell GrowthCell Lysis

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Ho, C. Y., Choy, C. H., Botelho, R. J. Radiolabeling and Quantification of Cellular Levels of Phosphoinositides by High Performance Liquid Chromatography-coupled Flow Scintillation. J. Vis. Exp. (107), e53529, doi:10.3791/53529 (2016).

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