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JoVE Journal Neuroscience
Quantifying Fish Swimming Behavior in Response to Acute Exposure of Aqueous Copper Using Computer Assisted Video and Digital Image Analysis
 

Quantifying Fish Swimming Behavior in Response to Acute Exposure of Aqueous Copper Using Computer Assisted Video and Digital Image Analysis

Article DOI: 10.3791/53477-v • 16:21 min • February 26th, 2016
February 26th, 2016 •
1, 1, 1, 1, 2
1Columbia Environmental Research Center, US Geological Survey, 2US Geological Survey

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Measuring the impacts of environmental contaminants on fish behavior is often subjective and challenging particularly when dealing with sublethal endpoints. We describe methods including video technology to quantify swimming behavior of early life stage white sturgeon (Acipenser transmontanus) during and after 96 hr acute exposures to various concentrations of copper.

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Quantify Fish Swimming Behavior Acute Exposure Aqueous Copper Computer Assisted Video Digital Image Analysis White Sturgeon Ecotoxicology Sub-lethal Exposure Contaminants Swimming Behavior Fish Species Amphibians Invertebrates Sensitivity Toxicity Organism Survival Behavioral Measurements Protocol Demonstration Holly Puglis Aqueous Reagent Grade Copper Two Sulfate Pentahydrate Diluter System Exposure Tanks Automated Syringe Dispenser Automated Pump Spike Volume Dilution Series Control Four-way Splitter Exposure Chambers Chiller
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