需要订阅 JoVE 才能查看此. 登录或开始免费试用。
测量的生理反应<em>果蝇</em>感觉神经元脂质费洛蒙使用实时钙成像
8,404 Views
•
07:25 min
•
April 29, 2016
DOI:
1Temasek Life Sciences Laboratory, 2Department of Biological Science,National University of Singapore, 3Bioimaging and Biocomputing Facility,Temasek Life Sciences Laboratory, 4Histology and Light Microscopy Core,Gladstone Institutes, 5Pacific Biosciences Research Center,University of Hawai’i at Mānoa
Summary
Automatically generated
The forelegs and proboscis of Drosophila contain a rich repertoire of gustatory sensory neurons. Here, we present a method using calcium imaging to measure physiological responses from sensory neurons in the foreleg and proboscis of live flies upon exogenous application of a gustatory pheromone.
Read Article
Cite this Article
Shankar, S., Calvert, M. E., Yew, J. Y. Measuring Physiological Responses of Drosophila Sensory Neurons to Lipid Pheromones Using Live Calcium Imaging. J. Vis. Exp. (110), e53392, doi:10.3791/53392 (2016).
Copy