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Chapters
- 00:05Title
- 01:14Enrichment of Whole Bruch’s Membrane
- 05:00Solubilization of Whole Enriched Bruch’s Membrane and Western Blot Analysis
- 06:34Enrichment of Macular Bruch’s Membrane
- 08:51Results: Histology and Protein Content of Bruch’s Membrane
- 09:35Conclusion
Identifying proteins specifically associated with Bruch’s membrane in human eyes is an important step in understanding the biochemical mechanisms behind eye diseases such as age-related macular degeneration. This protocol describes how to enrich this sheet of extracellular matrix for down-stream biochemical analysis.
