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하는 세포 칼슘의 형광 및 생물 발광 영상<sup> 2 +</sup> 노인 해마 신경 세포에서
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Fluorescence and Bioluminescence Imaging of Subcellular Ca2+ in Aged Hippocampal Neurons
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하는 세포 칼슘의 형광 및 생물 발광 영상<sup> 2 +</sup> 노인 해마 신경 세포에서

8,176 Views

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13:24 min

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December 01, 2015

DOI:

10.3791/53330-v

DOI:
10.3791/53330-v

•

13:24 min
December 01, 2015

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3 Views
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1Instituto de Biologìa y Genética Molecular,Consejo Superior de Investigaciones Cientìficas, 2Departamento de Bioquìmica y Biologìa Molecular y Fisiologìa,Universidad de Valladolid

Summary

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Intracellular Ca2+ remodeling in aging may contribute to excitotoxicity and neuron damage, processes mediated by Ca2+ overload. We aimed at investigating Ca2+ remodeling in the aging brain using fluorescence and bioluminescence imaging of cytosolic and mitochondrial Ca2+ in long-term cultures of rat hippocampal neurons, a model of neuronal aging.

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Calvo-Rodríguez, M., Villalobos, C., Nuñez, L. Fluorescence and Bioluminescence Imaging of Subcellular Ca2+ in Aged Hippocampal Neurons. J. Vis. Exp. (106), e53330, doi:10.3791/53330 (2015).

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