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Spatiotemporal Mapping of Motility in Ex Vivo Preparations of the Intestines
JoVE 杂志
生物学
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JoVE 杂志 生物学
Spatiotemporal Mapping of Motility in Ex Vivo Preparations of the Intestines

Spatiotemporal Mapping of Motility in Ex Vivo Preparations of the Intestines

DOI:
10.3791/53263-v

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12:00 min

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January 27, 2016

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, ,
1Department of Physiology and Biophysics,Virginia Commonwealth University, 2Department of Biology,Loyola University Maryland

Chapters

  • 00:05Title
  • 01:07Preparing the Tissues
  • 03:56Video Recording Calibration
  • 05:34General Experimental Procedure
  • 07:01Constructing Spatiotemporal Maps (ST-maps)
  • 09:14Interpreting Spatiotemporal Maps (ST-maps)
  • 11:05Conclusion

Summary

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Recently available video recording and spatiotemporal mapping (STmap) techniques make it possible to visualize and quantify both propagating and mixing patterns of intestinal motility. The goal of this protocol is to explain the generation and analysis of STmaps using the GastroIntestinal Motility Monitoring (GIMM) system.

Tags

Keywords: Spatiotemporal MappingIntestinal MotilityEx Vivo IntestinesIntestinal Wall MovementMotility PatternsDrug EffectsIntraluminal AgentsGuinea PigKrebs SolutionPolyethylene TubingGlass Tube CathetersOrgan BathModelers Clay

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