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JoVE Journal
Bioengineering

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A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
 

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Article DOI: 10.3791/53120-v 09:09 min November 23rd, 2015
November 23rd, 2015

章节

总结概括

Inter-cellular communication is critical for controlling various physiological activities within and outside the cell. This paper describes a protocol for measuring the spatio-temporal nature of single cell secretions. To achieve this, a multidisciplinary approach is used which integrates label-free nanoplasmonic sensing with live cell imaging.

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Keywords: Label-free Single Cell Secretions Localized Surface Plasmon Resonance Imaging (LSPRi) Protein Secretion Cell-to-cell Signaling Nanofabrication Biofunctionalization Binding Kinetics Live Cell Imaging
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