Journal
/
免疫与感染
/
Immunofluorescence to Monitor the Cellular Uptake of Human Lactoferrin and its Associated Antiviral Activity Against the Hepatitis C Virus
JoVE 杂志
免疫与感染
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 免疫与感染
Immunofluorescence to Monitor the Cellular Uptake of Human Lactoferrin and its Associated Antiviral Activity Against the Hepatitis C Virus

Immunofluorescence to Monitor the Cellular Uptake of Human Lactoferrin and its Associated Antiviral Activity Against the Hepatitis C Virus

DOI:
10.3791/53053-v

•

06:28 min

•

October 01, 2015

•

, ,
1Département de biochimie, Faculté de médecine et des sciences de la santé,Université de Sherbrooke

Chapters

  • 00:05Title
  • 01:11Cell Preparation and Fixation
  • 03:12Immunofluorescence Microscopy
  • 04:57Results: Cellular Uptake of Human Lactoferrin in Huh-7 Cells
  • 05:59Conclusion

Summary

自动翻译

自动翻译

The human lactoferrin (hLF) is a component of the immune system. In this study, immunofluorescence assays are used to demonstrate both the hepatocellular uptake of hLF and a qualitative reduction in the hepatitis C virus replication upon treatment with hLF.

Tags

ImmunofluorescenceFluorescence MicroscopyConfocal MicroscopyLactoferrinHepatitis C VirusCellular UptakeAntiviral Activity

Article

Embed

添加到播放列表

Usage Statistics

Related Videos

The Use of Carboxyfluorescein Diacetate Succinimidyl Ester (CFSE) to Monitor Lymphocyte Proliferation

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins

Assays for the Identification of Novel Antivirals against Bluetongue Virus

A Protocol for Analyzing Hepatitis C Virus Replication

In Vitro Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection

Development of a Hepatitis B Virus Reporter System to Monitor the Early Stages of the Replication Cycle

Using Zebrafish Models of Human Influenza A Virus Infections to Screen Antiviral Drugs and Characterize Host Immune Cell Responses

Generation of Escape Variants of Neutralizing Influenza Virus Monoclonal Antibodies

Overexpression and Purification of Human Cis-prenyltransferase in Escherichia coli

Detection of Antibodies That Neutralize the Cellular Uptake of Enzyme Replacement Therapies with a Cell-based Assay

Read Article