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Instillation and Fixation Methods Useful in Mouse Lung Cancer Research
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JoVE 杂志 医学
Instillation and Fixation Methods Useful in Mouse Lung Cancer Research

Instillation and Fixation Methods Useful in Mouse Lung Cancer Research

DOI:
10.3791/52964-v

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06:57 min

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August 31, 2015

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, ,
1Bloomberg School of Public Health, Environmental Health Sciences,Johns Hopkins University, 2Department of Medicine, Pulmonary Diseases and Critical Care Medicine,University of North Carolina School of Medicine

Chapters

  • 00:05Title
  • 01:51Lung Instillation
  • 03:34Lung Fixation
  • 04:55Measurement of the Fixed Lung Volume
  • 06:01Results: Instillation and Fixed Lung Volume Measurement
  • 06:21Conclusion

Summary

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The goal of this paper is to describe simple methods that will greatly aid in the setup and analysis of mouse lungs with lung cancer or other pathologies. We present 3 protocols to simply and reliably carry out lung instillations, fixation, and lung volume measurements.

Tags

Keywords: Mouse Lung Cancer ResearchTracheal InstillationLung FixationLung Volume MeasurementHistologic AnalysisStereological QuantificationLung StructureLung Tumor

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