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Production of Nurr-1 Specific Polyclonal Antibodies Free of Cross-reactivity Against Its Close Homologs, Nor1 and Nur77
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JoVE 杂志 生物学
Production of Nurr-1 Specific Polyclonal Antibodies Free of Cross-reactivity Against Its Close Homologs, Nor1 and Nur77

Production of Nurr-1 Specific Polyclonal Antibodies Free of Cross-reactivity Against Its Close Homologs, Nor1 and Nur77

DOI:
10.3791/52963-v

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12:30 min

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August 17, 2015

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, , , , ,
1Molecular Neurobiology Laboratory, Psychiatry,McLean Hospital, 2Department of Biochemistry, College of Medicine,Konyang University

Chapters

  • 00:05Title
  • 01:31Coupling of LBD Proteins to AminoLink Coupling Resin
  • 04:11Blocking Remaining Sites
  • 05:43Purification of Nurr1 Specific Antibodies
  • 07:21ELISA-based Analysis of Purified Anti-Nurr1 Antibody
  • 10:04Results: Pre-adsorption Against Cross-reactive Antigens Increases Specificity of Anti-Nurr1 Polyclonal Antibodies
  • 11:56Conclusion

Summary

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Here, we selectively target antibodies against a specific member of a highly conserved family of proteins by immunizing animals with their most divergent regions followed by removing cross reactive antibodies by pre-adsorption.

Tags

Nurr1NR4A2Nur77NR4A1NOR1NR4A3Polyclonal AntibodiesCross-reactivityDNA Binding DomainLigand-binding DomainParkinson’s DiseaseSchizophreniaManic DepressionHippocampusLearningMemoryAffinity ChromatographyProtein APre-adsorption

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