需要订阅 JoVE 才能查看此. 登录或开始免费试用。
Chapters
- 00:05Title
- 01:28Calcium-phosphate Transfection of HEK293T Cells
- 03:05Preparation of Cell Lysates for Co-immunoprecipitation
- 04:09Preparation of Immune Complexes and Protein G Beads
- 05:38Immunoprecipitation of the Immune Complexes by Protein G Beads and their Elution
- 08:00Results: Mx1 Interacts with Influenza A Virus Nucleoprotein
- 09:03Conclusion
This co-immunoprecipitation protocol allows to study the interaction between the influenza A virus nucleoprotein and the antiviral Mx1 protein in human cells. The protocol emphasizes the importance of N-ethylmaleimide for successful co-immunoprecipitation of Mx1 and influenza A virus nucleoprotein.
